Atga2ox8基因过表达载体构建及遗传转化植株表型分析

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August undefined, 2024

WebNov 14, 2014 · The tblastn program was run to identify the homologous gene sequences in all available switchgrass expressed sequence tag (EST) databases using the amino acid sequences of AtGA2ox8 , OsGA2ox5 (LOC_Os07g01340.1), OsGA2ox6 (LOC_Os04g44150.1) and OsGA2ox9 (LOC_Os02g41954.1). Phylogenetic trees and … WebOverexpression of both AtGA2ox7 and AtGA2ox8 caused dwarfing ( Figures 1C and 1D), indicating that the substrates for these enzymes are required for normal elongation …

Heterologous Expression of a Gibberellin 2-Oxidase Gene from

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CRISPR-Based Assessment of Gene Specialization in the …

WebApr 29, 2010 · Gibberellin 2-oxidase (GA 2-oxidase) plays very important roles in plant growth and development. In this study, the AtGA2ox8 gene, derived from Arabidopsis (Arabidopsis thaliana), was transformed and over-expressed in rapeseed (Brassica napus L.) to assess the role of AtGA2ox8 in biomass accumulation and lignification in plants. … WebOct 30, 2024 · Key message Using a fixed RIL population derived from a widely used foxtail millet backbone breeding line and an elite cultivar, we constructed a high-density bin map and identified six novel multi-environment effect QTLs and seven candidate genes for dwarf phenotype. Abstract Plant height is an important trait that determines tradeoffs between … WebWe identified two genes in Arabidopsis (AtGA2ox7 and AtGA2ox8), using an activation-tagging mutant screen, that encode 2beta-hydroxylases. GA levels in both activation-tagged lines were reduced... jesus manero maza

Full article: Analysis of regulatory elements in GA2ox, GA3ox and ...

Proper gibberellin localization in vascular tissue is required to ...

WebAtGA2ox7 and AtGA2ox8 are more similar to each other than to other proteins encoded in the Arabidopsis genome, indicating that they may constitute a separate class of GA … WebJul 13, 2024 · Both genes encode enzymes that have functional similarities to AtGA2ox7 and AtGA2ox8, which are class III GA 2-oxidases that 2β-hydroxylate C 20 -GAs. Previously unknown for GA 2-oxidases, AtGA2ox9 performs 2α-hydroxylation of C 19 -GAs and harbors putative desaturating activity of C 20 -GAs. lampiran se 55 tahun 2021WebWe found that CsGA2ox8, a gene whose product catalyzes the deactivation of bioactive GAs, was signiﬁcantly upregulated in treatment X1 compared to treatment X2. This expression difference was conﬁrmed by quantitative reverse … jesus manso uam

"WebApr 13, 2024 · 30DayWeather Long Range Weather Forecasts predict ideal conditions for a storm.; A Risky Day is not a direct prediction of precipitation (Rain/Snow) but instead a … " - Atga2ox8基因过表达载体构建及遗传转化植株表型分析

Atga2ox8基因过表达载体构建及遗传转化植株表型分析

Proper gibberellin localization in vascular tissue is required to ...

WebWe identified two genes in Arabidopsis (AtGA2ox7 and AtGA2ox8), using an activation-tagging mutant screen, that encode 2beta-hydroxylases. GA levels in both activation … WebThe two GA2ox genes – AtGA2ox8 and AtGA2ox2 – were chosen because the enzymes that they encode catabolize early precursors of GA (C 20 -GA) or late precursors and bioactive GAs (C 19 -GA), respectively ( Schomburg et al., …

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Web过表达载体主要是将目的基因的编码区（CDS）构建到相应的质粒或者病毒载体中，达到在目的基因过量表达的作用。过表达载体的主要元件：Promoter—gene—linker—Fluorescent gene—抗药筛选gene。花椰菜花叶病毒 (CaMV) 35S是组成型启动子，具多种顺式作用元件。其转录起始位点上游－343～－46bp是转录增强区，－343～－208和－208～－90bp … WebJun 3, 2024 · Gibberellin (GA) functions as an essential natural regulator of growth and development in plants. For each step of the GA metabolic pathway, different copy numbers can be found in different species, as is the case with the 13 genes across four enzymatic steps in rice ( Oryza sativa ).

WebAug 3, 2010 · 2012-03-26 基因的克隆,转导,表达,在对目的基因pcr时,为什么一些人设... 8 2011-08-24 你好，请问我是要提取一段目的基因，后续要表达蛋白，在引物设计... 1 2012 … WebNov 1, 2013 · Morphological alterations by overexpression of the GA2ox gene have already been reported for various plant species: transgenic A. thaliana and N. tabacum plants containing AtGA2ox7 or AtGA2ox8 from A. thaliana exhibited dwarf phenotypes (Schomburg et al., 2003); transgenic Solanum melanocerasum and S. nigrum plants …

WebJan 27, 2014 · The overexpression of AtGA2ox7 and AtGA2ox8 produce a dwarf phenotype with reduced GA levels, while ectopic expression of AtGA2ox7 and AtGA2ox8 in transgenic tobacco ( Nicotiana tabacum) leads to a dwarf phenotype [22]. A similar phenotype was also observed in rice overexpressing OsGA2ox6 [25]. WebDec 14, 2010 · Gibberellins (GAs) are endogenous hormones that play an important role in regulating plant stature by increasing cell division and promoting seed germination. The GA2-oxidase gene from Arabidopsis thaliana (AtGA2ox8) was introduced into Brassica napus L. by Agrobacterium-mediated floral-dip transformation with the aim of decreasing …

WebMar 20, 2014 · Gibberellin (GA), a plant hormone, is involved in many aspects of plant growth and development both in vegetative and reproductive phases. GA2-oxidase plays a key role in the GA catabolic pathway to reduce bioactive GAs. We produced transgenic Arabidopsis plants expressing GA2-oxidase 4 (AtGA2ox4) under the control of a …

WebApr 11, 2024 · Jo-Anne Theresa Glassock. July 4, 1946 - March 20, 2024 Dearly beloved Jo-Anne Theresa Glassock passed away on March 20, at the age of 76, in Laguna … lampiran seminar ptkWebJan 27, 2014 · The overexpression of AtGA2ox7 and AtGA2ox8 produce a dwarf phenotype with reduced GA levels, while ectopic expression of AtGA2ox7 and AtGA2ox8 in … jesus manger imagesWebAtGA2ox7 and AtGA2ox8 are more similar to each other than to other proteins encoded in the Arabidopsis genome, indicating that they may constitute a separate class of GA-modifying enzymes. Indeed, enzymatic assays demonstrated that AtGA2ox7 and AtGA2ox8 both perform the same GA modification: 2beta-hydroxylation of C20-GAs but not of C19 … lampiran setelah daftar pustakaWebAug 1, 2024 · GA2-oxidases (GA2oxs) are a class of 2-oxoglutarate-dependent dioxygenases that regulate the deactivation of bioactive GAs. In this study, we investigated the phylogeny and domain structures of the seven GA2ox genes present in the Arabidopsis thaliana genome. jesus mantecaWebJan 1, 2024 · The homolog of CsGA2ox8 in Arabidopsis is AtGA2ox8, whose product functions in hydroxylating the C 20-GA precursors GA 12 and GA 53 15. In this study, consistent with the results from overexpression of AtGA2ox8 15, GA 12 and GA 53 levels dramatically decreased in OE8.1, suggesting that CsGA2ox8 has a function similar to … jesus manna from heaven jesus mantraBoth genes encode enzymes that have functional similarities to AtGA2ox7 and AtGA2ox8, which are class III GA 2-oxidases that 2β-hydroxylate C20-GAs. Previously unknown for GA 2-oxidases, AtGA2ox9 performs 2α-hydroxylation of C19-GAs and harbors putative desaturating activity of C20-GAs. jesus manifesto luke 4