WebHis Tag: Products. Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His … WebAll lanes : Anti-6X His tag® antibody [HIS.H8] (ab18184) at 1/1000 dilution Lane 1 : 6x His-tagged protein expressed in BL21 (pET15b) at 1 µg Lane 2 : 6x His-tagged protein …
Labeling Antibodies with N-Hydroxysuccinimide-Long Chain (NHS …
Web31-1048-02. Clone Name. RM146. Product Data Sheet. Biotin Anti-His Tag Rabbit Monoclonal Antibody Data Sheet RM146. Specificity. RM146 reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to … WebRabbit anti-His Tag Biotinylated Antibody, 50 ug. Immunostaining (recommended work dilution= 1:200), Western Blotting (recommended work dilution= 1:1000), Immunoprecipitation (recommended work dilution= 1:200), (see image1) His Western Blot analysis of 293 cells transfected from with His-tagged vector (2) and untransfected … biontech philippines
What are Biotinylated Antibodies? - News-Medical.net
WebPolyclonal Antibody for studying His-Tag. Cited in 149 publications. Validated for Western Blotting, Immunoprecipitation. ... Secondary Antibody Conjugated to HRP: Anti-rabbit IgG, HRP-linked Antibody ... Loading of … WebHis-tags. Molecular Weight: 0.2–1.6 kDa. 6x-His tag is 0.8 kDa. Size: 2–10 histidine residues Tag location: C- or N- terminals, or internal. Affinity Resin: transition metal ions, usually Ni 2+ Applications: protein purification Strengths: Small size so lower possibility of affecting fusion protein functionality. Doesn’t cause inclusion bodies. Limitations: Can … WebThis antibody, which contains a mouse IgG2a isoform, was produced as a recombinant protein expressed in HEK293-derived cells using an engineered construct based on a … daily wiccan rituals