Web1.2 Gently shake the bottle of Glutathione Sepharose 4B to resus-pend the gel. 1.3 Use a pipet to remove sufficient slurry for use and transfer to an appropriate container/tube. Dispense 1.33 ml of the original Glutathione Sepharose 4B slurry per ml of bed volume required. One ml of drained gel is capable of binding at least 8 mg of recombinant ... WebGSTrap™ HP 1-mL and 5-mL columns are prepacked with Glutathione Sepharose High Performance resin, which provides high-resolution purifications, thus a more concentrated sample. GST-tagged proteins are purified from pretreated bacterial lysates by affinity chromatography using immobilized glutathione, such as Glutathione Sepharose High ...

GSTrap™ HP Columns Cytiva

WebGlutathione Sepharose 4B required for your application. Glutathione Sepharose 4B as supplied is approximately a 75% slurry. The following procedure results in a 50% slurry. • Gently shake the bottle of Glutathione Sepharose 4B to resuspend the gel. • Use a pipet to remove sufficient slurry for use and transfer to an appropriate container/tube. http://www.proteinguru.com/protocols/GST%20column.pdf imdb invisible man

NAP1-mCherry - ResearchGate

WebInstructions for Use. Glutathione Sepharose™ 4B is designed for the single-step purification of glutathione S-transferases, glutathione-dependent proteins and … Web易于与注射器、泵或层析系统配合使用。. GSTrap™ HP 1-mL 和 5-mL 层析柱预填充了 Glutathione Sepharose High Performance 填料。. 利用该填料可实现高分辨率纯化，从而提高样品浓度。. 使用螯和谷胱甘肽的层析柱（例如 Glutathione Sepharose High Performance），利用亲和层析法从 ... WebGlutathione Sepharose 4B (Cytiva) Superdex 200 increase 10/300 column (Cytiva) ... Cytiva) pre-equillibrated with a SEC buffer containing 20 mM Tris-HCl, pH 7.4, 150 mM NaCl, and 1 mM DTT. ... imdb invisible man 2020