Phospho chk2 antibody
WebCHK2 Antibodies Antibodies that detect CHK2 can be used in several scientific applications, including Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry … WebJun 30, 2024 · Membranes were incubated in 5% nonfat milk for 45 min at room temperature and then incubated for 18 hours at 4°C with primary antibodies against the following molecules diluted in 5% nonfat milk: p21, p27, phospho-Chk1, Chk1, phospho-Chk2, Chk2, ZEB1, ZEB2, p53, and γH2AX (table S10).
Phospho chk2 antibody
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WebAll lanes : Anti-Chk2 (phospho T387) antibody (ab55319) at 1/500 dilution Lane 1 : Jurkat cell lysate Lane 2 : Jurkat cell lysate with the immunising phosphopeptide. Predicted band … WebCHEK2 (Ab-516) Antibody ( ELISA,WB ) ( CSB-PA074742 ) Phospho-CHEK2 (Thr387) Antibody ( ELISA,WB ) ( CSB-PA983913 ) Phospho-CHEK2 (Thr383) Antibody ( ELISA,WB,IF ) ( CSB-PA159350 ) CHEK2 (Ab-68) Antibody ( ELISA,WB,IHC ) ( CSB-PA884585 ) More>> CHEK2 Proteins
WebThe Phospho-Chk1/2 Antibody Sampler Kit offers an economical means to evaluate the phosphorylation status of Chk1 and Chk2 on multiple residues. The kit contains enough … WebKey features and details Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply APC Rabbit monoclonal [Chk2T68-D12] to Chk2 (phospho T68) Suitable for: Flow Cyt Reacts with: Human Conjugation: APC. Ex: 645nm, Em: 660nm Related conjugates and formulations FITC PE Unconjugated
WebWestern blot - Phospho-Chk2-T68 Rabbit pAb (AP0590) Western blot analysis of extracts of HeLa cells, using Phospho-Chk2-T68 antibody (AP0590). HeLa cells were treated by UV for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. WebMar 25, 2024 · Flow cytometric analysis of phospho-Chk2 pThr68 on untreated (blue histogram) or UV-treated (green histogram) HeLa cells. Cells were harvested, fixed with formaldehyde, permeabilized with methanol, and incubated with a Phospho-Chk2 pThr68 polyclonal antibody (Product # PA5-17818) at a 1:800 dilution for 1 hour at room …
WebThe oncogenic cytoplasmic p21 contributes to cancer aggressiveness and chemotherapeutic failure. However, the molecular mechanisms remain obscure. Here, we show for the first time that cytoplasmic p21 mediates 5-Fluorouracil (5FU) resistance by shuttling p-Chk2 out of the nucleus to protect the tumor cells from its pro-apoptotic …
how to share large files by emailWebHuman Phospho-Chk2 (T68) Antibody Cat # AF1626 (2) Citations (7) Human/Mouse/Rat 14-3-3 sigma Antibody Cat # AF4424 (1) Citations (1) Human Phospho-p53 (S15) Antibody Cat # AF1043 (3) Citations (7) Human Ki67/MKI67 Antibody Cat # MAB7617 (2) Citations (5) Human p21/CIP1/CDKN1A Antibody Cat # AF1047 (4) Citations (3) notion formula day of the weekWebPVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-CDC2 (Y15) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF888), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-CDC2 (Y15) at approximately 34 kDa (as indicated). notion formula days between two datesWebPhospho-CHK2 (Ser19) Polyclonal Antibody Catalog Number PA5-17731 Product Data Sheet Product Specific Information ... surrounding pSer19 of human Chk2 Conjugate Unconjugated Form Liquid Purification Antigen affinity chromatography … notion formula another databaseWebChk2 is the mammalian orthologue of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases (1-3). The amino-terminal domain of Chk2 contains a series of seven … notion form to databaseWebImmunocytochemistry/ Immunofluorescence: Chk2 [p Thr68] Antibody [NBP3-13076] - Chk2 (phospho Thr68) antibody detects Chk2 (phospho Thr68) protein at nucleus by immunofluorescent analysis. Sample: Mock and treated HeLa ...read more how to share large files with othersWebBackground: The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA … how to share large files with china